https://safirsoft.com The two research teams independently used the space to measure biodiversity

Collecting environmental DNA in water is becoming common - can we do it in air now? Vacuum cleaner and filter. He tested them and eventually landed on a water vacuum that was too good for his purposes. The rest weren't completely cut out - they had good suction, but the second I inserted the filter, their power supply malfunctioned. “It just dies, and then the engine overheats too much, and that was very difficult,” Lingard said.

All of these experiments were conducted for an interesting case, which may have seemed obvious in the past but could have valuable environmental applications. In short, Lingard and other researchers on his team were looking for a way to collect environmental DNA (eDNA) from the air to measure biodiversity or to search for rare or invasive species.

out of the air. "We had no idea how to collect DNA from the air," Kristen Bowman told Ars. Bowman is an associate professor at the Globe Institute and one of the research associates.

As it turns out, you can get RNA out of the air by suctioning it through a vacuum (or something similar.) Collect. ), attach it to a filter and analyze it. Bowman, Lingard, and other researchers recently published their findings in Current Biology. The publication of this study coincides with a different study that shows largely the same results using a slightly different method developed by a team in the UK and Canada.

In the past, attempts have been made to measure biodiversity or checking the presence of a species was a snoring task that often involved mounting a camera or going out and waiting for the species to be identified. However, researchers are recently using eDNA for this purpose, as it may be easier. “According to an article last February, the most common form of eDNA testing involves filtering the surrounding water through a membrane and examining the accumulated material — often bits of skin, feces, mucus, etc., that carry DNA,” Bowman says. It surrounds everything on Earth, just as water surrounds everything in a lake or ocean, however, these two substances describe something that has not generally been done before: eDNA measurement from air: This is not an entirely new concept; A study last year used air, water, and soil to identify large brown bats, but Danish researchers think they have something to do with it.

“I felt good about it. I just knew I had to do this study.” Bowman said he remembers the first grant. He wrote it for the project, which was rejected.Advertisement

Proof of Concept

In 2019, Baumann and Lingard's second attempt was made to receive a grant. To test their ideas, the team was fitted with a vacuum cleaner at the Copenhagen Zoo and, at the suggestion of one of the co-authors, were fitted with two fans.The fans were similar to the fans in laptops, but had a 3D-printed chamber so that the filters could be attached to.Lingard tested several filters before landing on the A-class filters. F8 which is good at collecting and preserving particles, from there the team walked around the zoo and collected samples from three different Kurdish collection areas: a stable containing an okapi and a tiger An outdoor storage area and inside a “rainforest house” where birds, reptiles, sloths, etc. appeared y that. Vacuum cleaners suck air from these areas and trap bits of animal droppings in filters or if the water is vacuumed. . In the laboratory, the water can also be filtered.

The laboratory is well cleaned and has strict entry rules to prevent sample contamination. The team also collected air samples from the lab to learn about DNA in the environment.

At first, they weren't sure what kind of DNA they would find, if any. Team members identified 49 different species, from rhinos to guppies, in the rainforest chamber, from 40 samples they took. Each sample contained DNA between six and 21. Some of the species discovered — such as crabs and red squirrels — were not even zoo animals. “We were completely surprised and surprised and shocked that we fell off the chair,” Bowman said.



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